| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | To improve visibility and measure gene expression future experiments, each homologue geneinsert was cloned into a pEGFP-C2 vector. The Genscript gene products were provided in a pUC57 vector. To release the gene-insert from the pUC57 vector, 5 ug of the gene-insert-pUC57 vector was digested with New England Biolabs (NEB) restriction enzymes BamHI (1.5 µL) and XhoI (1.5 µL). The pEGFP-C2 vector (5 ug) was digested with NEB BgIII and SalI restriction enzymes. To ensure an optimal digestion environment, 10x NEB Buffer 3.1 (7.5 µL), 100x Bovine Serum Albumin (BSA) (0.75 µL), Shrimp Alkaline Phosphatase (SAP) (1 µL), and ddH2O (used to increase final reaction volume to 75 uL) were included in each digestion reaction. | Get A Quote |
Kaposi sarcoma is a type of lymphatic and skin cancer that is caused by Kaposi Sarcomaassociated virus (KSHV). KSHV ORF45 is classified as an immediate early gene that has multifunctional roles. One of its major functions is to activate the extracellular signal-regulated kinase (ERK) and p90 ribosomal kinase 1 and 2 (RSK1/2) in the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway. It has been discovered that KSHV ORF45 can activate both the ERK and RSK which promote the reactivation of KSHV. There is limited evidence that observes the behavior of all KSHV ORF45 homologs. Therefore, we are interested in studying the functional conservativeness within gammaherpesvirus ORF4... More
