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Molecular characterization and functional expression of voltage-gated sodium channel variants in Apolygus lucorum (Meyer-Dür)

Pest Manag Sci. 2020; 
Zhang K, Chen M, Wang H, Duan W, Wang Q, Li F, Deng D, Bandason E, Wu S.
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Molecular Biology Reagents … Professor Ke Dong, Department of Entomology, Michigan State University) The PCR products (Fig SB) were determined by the Genscript biotechnology company (China) The plasmid of AlNav-PGH19 (Fig SC) were detected by double enzyme digestion … Get A Quote

摘要

Apolygus lucorum (Meyer-Dür) is a serious worldwide agricultural pest, especially for Bt cotton in China. Pyrethroids, neonicotinoids and organophosphates are the most effective insecticides to control piercing and sucking insects, including A. lucorum. The voltage-gated sodium channel (Nav ) is major target site of pyrethroids. Extensive alternative splicing and RNA editing, two major post-transcriptional mechanisms, contribute to generate different functional sodium channel variants. In our research, we characterized the sodium channel variants of A. lucorum.,In this study, we isolated numerous sodium channel variants that cover the entire coding region of the VGSC gene from A. lucorum. All clones could be g... More

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