| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … Prof Anett Schallmey (University of Braunschweig) CYP107D1 was purchased from GenScript as codon-optimized synthetic gene based on the GenBank entry Q59819 and was subcloned into pET-28a vector All chemicals … | Get A Quote |
Regio- and stereoselective hydroxylation of lithocholic acid (LCA) using CYP107D1 (OleP), a cytochrome P450 monooxygenase from the oleandomycin synthesis pathway of Streptomyces antibioticus.,Co-expression of CYP107D1 from S. antibioticus and the reductase/ferredoxin system PdR/PdX from Pseudomonas putida was performed in Escherichia coli whole cells. In vivo hydroxylation of LCA exclusively yielded the 6β-OH product murideoxycholic acid (MDCA). In resting cells, 19.5% of LCA was converted to MDCA within 24 h, resulting in a space time yield of 0.04 mmol L-1 h-1. NMR spectroscopy confirmed the identity of MDCA as the sole product.,The multifunctional P450 monooxygenase CYP107D1 (OleP) can hydroxylate LCA, fo... More
