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Growth and Purification of Vaccinia Virus Stocks for MPM Imaging

Methods Mol Biol. 2019;

Reynoso GV, Shannon JP, Americo JL, Gibbs J, Hickman HD.

Products/Services Used	Details	Operation
Gene Synthesis	The authors have reliably used Operon and GenScript to produce constructs for the expression of tagged, codon-optimized vaccinia genes.	Get A Quote

摘要

This chapter provides methods for the propagation, purification, and titration of vaccinia virus (VACV) and the highly attenuated strain-modified vaccinia Ankara (MVA). Additionally, we provide information on VACV recombinants we have used for intravital imaging with multiphoton excitation.