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Design and tailoring of an artificial DNA scaffolding system for efficient lycopene synthesis using zinc-finger-guided assembly.

J Ind Microbiol Biotechnol. 2020; 
Xu X, Tian L, Tang S, Xie C, Xu J, Jiang L.
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Molecular Biology Reagents … study were obtained from Lee et al [16], and were synthesized and inserted into pUC57 by GenScript (China) to form the plasmids pUC57-ADB1, pUC57-ADB2, and pUC57-ADB3 The artificial DNA sequences 5′-CAA GCT … Get A Quote

摘要

A highly efficient lycopene production system was constructed by assembling enzymes fused to zinc-finger motifs on DNA scaffolds in vitro and in vivo. Three key enzymes of the lycopene synthesis pathway, geranylgeranyl diphosphate synthase, phytoene synthase, and phytoene desaturase, were fused with zinc-finger proteins, expressed and purified. Recombinant plasmids of the pS series containing DNA scaffolds that the zinc-finger proteins can specifically bind to were constructed. In the in vitro system, the production efficiency of lycopene was improved greatly after the addition of the scaffold plasmid pS231. Subsequently, the plasmid pET-AEBI was constructed and introduced into recombinant Escherichia coli BL21... More

关键词

DNA scaffold; Lycopene; Multi-enzyme regulation; Zinc-finger protein