Apelin is a peptide that plays a role in physiological processes such as angiogenesis, apoptosis and proliferation. The aim of this study was to investigate the role of capsaicin-sensitive afferent neurons and vagus in effect of apelin against ischemia/reperfusion (I/R)-injury. Experimental groups were as 1)control, 2)I/R, 3)apelin+I/R, 4)vagotomy+I/R, 5)vagotomy+apelin+I/R, 6)capsaicin+I/R; 7)capsaicin+apelin+I/R, 8)lorglumide+I/R and 9)lorglumide+apelin+I/R. To test the potential gastroprotective effect of apelin-13, apelin-13 (2mg/kg, i.v.) was administered just before both ischemia and reperfusion. Vagotomy was performed one week before I/R in the vagotomized groups; capsaicin (125mg/kg, s.c.) was administr... More
Apelin is a peptide that plays a role in physiological processes such as angiogenesis, apoptosis and proliferation. The aim of this study was to investigate the role of capsaicin-sensitive afferent neurons and vagus in effect of apelin against ischemia/reperfusion (I/R)-injury. Experimental groups were as 1)control, 2)I/R, 3)apelin+I/R, 4)vagotomy+I/R, 5)vagotomy+apelin+I/R, 6)capsaicin+I/R; 7)capsaicin+apelin+I/R, 8)lorglumide+I/R and 9)lorglumide+apelin+I/R. To test the potential gastroprotective effect of apelin-13, apelin-13 (2mg/kg, i.v.) was administered just before both ischemia and reperfusion. Vagotomy was performed one week before I/R in the vagotomized groups; capsaicin (125mg/kg, s.c.) was administrated two weeks before I/R in the capsaicin-treated groups and lorglumide (5mg/kg, i.p.) was administered 30min before I/R in lorglumide-treated groups. After I/R, variety parameters in gastric tissue were analyzed. cfos expression determined in brainstem samples. In I/R group lesion index, myeloperoxidase activity, lipid peroxidation, nitric oxide and tumor necrosis factor-α increased; mucosal blood flow, prostaglandin-E2 and calcitonin gen-related peptide were decreased. Apelin prevented damaging effects of I/R and increased cfos expression in brainstem areas. Vagotomy, capsaicin and lorglumide largely eliminated gastroprotective effects of apelin-13. This study showed that sensory nerves and vagus play regulatory roles in apelin-induced gastroprotection. Cholecystokinin may play a role in the effect of apelin through sensory neurons.
