| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … After selecting positive clones, they were sent to Nanjing GenScript Biotech Corp. (Nanjing, China) for sequencing. In order to verify the accuracy of the full-length sequence of the gene we designed primers outside the ORF and then performed PCR amplification verification … | Get A Quote |
Cryptomeria fortunei, also known as the Chinese cedar, is an important timber species in southern China. The primary component of its woody tissues is lignin, mainly present in secondary cell walls. Therefore, continuous lignin synthesis is crucial for wood formation. In this study, we aimed to discover key genes involved in lignin synthesis expressed in the vascular cambium of C. fortunei. Through transcriptome sequencing, we detected expression of two genes, 4CL and CCoAOMT, known to be homologous to enzymes involved in the lignin synthesis pathway. We studied the function of these genes through bioinformatics analysis, cloning, vascular cambium expression analysis, and transgenic cross-species functional val... More
