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TLR5 Ligand-Secreting T Cells Reshape the Tumor Microenvironment and Enhance Antitumor Activity.

Cancer Res. 2015; 
Geng D#, Kaczanowska S#, Tsai A, Younger K, Ochoa A, Rapoport AP, Ostrand-Rosenberg S, Davila E,.
Products/Services Used Details Operation
Custom Vector Construction The sequence was synthesized by GenScript, confirmed by DNA sequencing and then cloned into retroviral vector pMSGV1-MART-1-TCR, downstream of MART-1 TCR sequence, using an IRES sequence upstream of flagellin. Get A Quote

摘要

The tumor microenvironment counters antitumor T-cell responses, in part, by blunting their activation and infiltration. Ligands that engage Toll-like receptors (TLR) on T cells and antigen-presenting cells can act as potent immune adjuvants. In this study, we show how tumor-reactive T cells engineered to secrete bacterial flagellin, a TLR5 ligand (TLR5L), can engender a costimulatory signal that augments antitumor activity. Human T cells engineered to express TLR5L along with DMF5, a T-cell receptor that recognizes the melanoma antigen MART-127-35 (DMF5(TLR5L) T cells), displayed increased proliferation, cytokine production, and cytolytic activity against melanoma cells. In a xenogenetic model, adoptive transfe... More

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