Products/Services Used | Details | Operation |
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Custom Vector Construction> | miR-1SP construct was generated in the Han laboratory by introducing 10 repetitive miRNA complementary sequences (50 -GGTACGTTTAGCGTAAGTTAT-30 synthesized by GenScript) separated by four nucleotide linkers 50 -CGCG-30 into the pUAST vector. | Get A Quote |
Although the impact of microRNAs (miRNAs) in development and disease is well established, understanding the function of individual miRNAs remains challenging. Development of competitive inhibitor molecules such as miRNA sponges has allowed the community to address individual miRNA function in vivo. However, the application of these loss-of-function strategies has been limited. Here we offer a comprehensive library of 141 conditional miRNA sponges targeting well-conserved miRNAs in Drosophila. Ubiquitous miRNA sponge delivery and consequent systemic miRNA inhibition uncovers a relatively small number of miRNA families underlying viability and gross morphogenesis, with false discovery rates in the 4-8% range. In ... More