| Products/Services Used | Details | Operation |
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| Gene Synthesis | Next, in order to generate intestinal organoids, spheroids were cultured by floating with Advanced DMEM/F12 (Thermo Fisher Scientific) containing 3% Matrigel, 200 ng/mL R-Spondin1 (Noves), 100 ng/mL Noggin (GenScript), and 100 ng/mL epidermal growth factor (EGF, GenScript) with 2 mM L-Gln, N2 supplement (Thermo Fisher Scientific), B27 serum-free Page 7 of 40 7 S t e m e C l l s d n a D p o e v e l m n e t E f f i c i n e t e n e g r a t i o n o f y c o n m u g o l s m n o y e k i u d n c u p d e l r i o p t n e t t s e m c e l l - e d r i d e v i n t e t s i a n l o r d o n a g i s w i t a h p h r m c a o k i e n t i c f n u c t i n o s ( O D : I 9 8 0 1 0 1 . | Get A Quote |
In preclinical studies, the cynomolgus monkey (CM) model is frequently used to predict the pharmacokinetics of drugs in the human small intestine, because of its evolutionary closeness to humans. Intestinal organoids that mimic the intestinal tissue have attracted attention in regenerative medicine and drug development. In this study, we generated intestinal organoids from CM induced pluripotent stem (CMiPS) cells and analyzed their pharmacokinetic functions. CMiPS cells were induced into the hindgut; then, the cells were seeded on microfabricated culture vessel plates to form spheroids. The resulting floating spheroids were differentiated into intestinal organoids in a medium containing small-molecule compound... More
