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Functional dissection of NEAT1 using genome editing reveals substantial localization of the NEAT1_1 isoform outside paraspeckles.

RNA. 2017; 
Li R,, Harvey AR,, Hodgetts SI,, Fox AH,.
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Custom Vector Construction org on March 23, 2017 - Published by Cold Spring Harbor Laboratory Press Ruohan LI, page 20 396 AcGFP were incorporated into a pUC57 vector for propagation by supplier (Genscript). Get A Quote

摘要

Large numbers of long noncoding RNAs have been discovered in recent years, but only a few have been characterized. NEAT1 (nuclear paraspeckle assembly transcript 1) is a mammalian long noncoding RNA that is important for the reproductive physiology of mice, cancer development, and the formation of subnuclear bodies termed paraspeckles. The two major isoforms of NEAT1 (3.7 kb NEAT1_1 and 23 kb NEAT1_2 in human) are generated from a common promoter and are produced through the use of alternative transcription termination sites. This gene structure has made the functional relationship between the two isoforms difficult to dissect. Here we used CRISPR-Cas9 genome editing to create several different cell lines: tota... More

关键词

CRISPR genome editing; DBHS proteins; NEAT1; long noncoding RNAs; paraspeckles