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Custom Vector Construction> | For generation of shRNA constructs against mouse 11-HSD1, a small DNA insert (76-bp 5=-Mlu ACGCGTCGTTGAGTTCAT- tccaactcgag XhoI-3=) encoding a short-hairpin RNA (shRNA) target- CACAGCGACTGTtgatatccgCAGTCGCTGTGATGAACTCAAttttt- ing mouse 11-HSD1 (Gene bank: S75207) and scrambled shRNA was obtained from GenScript (SA Biosciences, Frederick, MD) and cloned into adenoviral shuttle vector pRNATin-H1. | Get A Quote |
Long-term glucocorticoid exposure increases the risk for developing type 2 diabetes. Prereceptor activation of glucocorticoid availability in target tissue by 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) coupled with hexose-6-phosphate dehydrogenase (H6PDH) is an important mediator of the metabolic syndrome. We explored whether the tissue-specific modulation of 11β-HSD1 and H6PDH in adipose tissue mediates glucocorticoid-induced insulin resistance and lipolysis and analyzed the effects of 11β-HSD1 inhibition on the key lipid metabolism genes and insulin-signaling cascade. We observed that corticosterone (CORT) treatment increased expression of 11β-HSD1 and H6PDH and induced lipase HSL and ATGL with s... More