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11β-Hydroxysteroid dehydrogenase type 1 shRNA ameliorates glucocorticoid-induced insulin resistance and lipolysis in mouse abdominal adipose tissue.

Am J Physiol Endocrinol Metab. 2015; 
Wang Y, Yan C, Liu L, Wang W, Du H, Fan W, Lutfy K, Jiang M, Friedman TC, Liu Y.
Products/Services Used Details Operation
Custom Vector Construction For generation of shRNA constructs against mouse 11␤-HSD1, a small DNA insert (76-bp 5=-Mlu ACGCGTCGTTGAGTTCAT- tccaactcgag XhoI-3=) encoding a short-hairpin RNA (shRNA) target- CACAGCGACTGTtgatatccgCAGTCGCTGTGATGAACTCAAttttt- ing mouse 11␤-HSD1 (Gene bank: S75207) and scrambled shRNA was obtained from GenScript (SA Biosciences, Frederick, MD) and cloned into adenoviral shuttle vector pRNATin-H1. Get A Quote

摘要

Long-term glucocorticoid exposure increases the risk for developing type 2 diabetes. Prereceptor activation of glucocorticoid availability in target tissue by 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) coupled with hexose-6-phosphate dehydrogenase (H6PDH) is an important mediator of the metabolic syndrome. We explored whether the tissue-specific modulation of 11β-HSD1 and H6PDH in adipose tissue mediates glucocorticoid-induced insulin resistance and lipolysis and analyzed the effects of 11β-HSD1 inhibition on the key lipid metabolism genes and insulin-signaling cascade. We observed that corticosterone (CORT) treatment increased expression of 11β-HSD1 and H6PDH and induced lipase HSL and ATGL with s... More

关键词

11β-hydroxysteroid dehydrogenase type 1; adipose tissue; glucocorticoid; hexose-6-phosphate dehydrogenase; insulin signaling; lipolysis; short-hairpin RNA