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Glutathione-complexed [2Fe-2S] clusters function in Fe-S cluster storage and trafficking.

J Biol Inorg Chem. 2016; 
Fidai I,, Wachnowsky C,, Cowan JA,,.
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Custom Vector Construction J Biol Inorg Chem Materials and methods Materials The gene for Saccharomyces cerevisiae S288c glutar- edoxin 3 (Grx3) in pET28b(+) vector was ordered from GenScript. Get A Quote

摘要

Glutathione-coordinated [2Fe-2S] complex is a non-protein-bound [2Fe-2S] cluster that is capable of reconstituting the human iron-sulfur cluster scaffold protein IscU. This complex demonstrates physiologically relevant solution chemistry and is a viable substrate for iron-sulfur cluster transport by Atm1p exporter protein. Herein, we report on some of the possible functional and physiological roles for this novel [2Fe-2S](GS4) complex in iron-sulfur cluster biosynthesis and quantitatively characterize its role in the broader network of Fe-S cluster transfer reactions. UV-vis and circular dichroism spectroscopy have been used in kinetic studies to determine second-order rate constants for [2Fe-2S] cluster transf... More

关键词

Glutaredoxin; Glutathione; Iron-sulfur cluster; Isa; IscU