Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction> | Expression-optimized ORFs for SFVmac (NC_010819), SFVagm (NC_010820), SFVora (AJ544579), SFVmar (GU356395), SFVsqu (GU356394), SFVspm (EU010385) and BFV (NC_001831) were synthesized by Genscript (Piscataway, USA) and subc- loned into the CMV-intron A driven eukaryotic expression vector pczi (Heinkelein et al. | Get A Quote |
Foamy viruses (FV) are retroviruses that are widely distributed in primate and non-primate animal species. We tested here FV with capsids of simian and non-simian origin for sensitivity to interferon-β (IFN-β). Our data show significant inhibition of FV by IFN-β early in infection of human HOS and THP-1 but not of HEK293T cells. The post-entry restriction of FV was not mediated by the interferon-induced MxB protein that was recently identified as a capsid-interacting restriction factor targeting Human immunodeficiency virus (HIV) before integration. Neither the ectopic expression of MxA or MxB in HEK293T cells nor the lack of MxB expression in CRISPR/CAS MxB THP-1 knockout cells impacted the infection of the... More