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| Peptide Synthesis | Cells were then washed and incubated with gp100 short peptide (10 μ M gp100(280–288); GenScript) or long peptide (100 μ M gp100(272–300); JPT) in combination with T cell receptor stimulation mix (4 μ g/ml R-848 and poly(I:C); Enzo Life Sciences) for 4 hours in X-vivo medium at 37 °C. | Get A Quote |
Dendritic cells (DCs) present foreign antigen in major histocompatibility complex (MHC) class I molecules to cytotoxic T cells in a process called cross-presentation. An important step in this process is the release of antigen from the lumen of endosomes into the cytosol, but the mechanism of this step is still unclear. In this study, we show that reactive oxygen species (ROS) produced by the NADPH-oxidase complex NOX2 cause lipid peroxidation, a membrane disrupting chain-reaction, which in turn results in antigen leakage from endosomes. Antigen leakage and cross-presentation were inhibited by blocking ROS production or scavenging radicals and induced when using a ROS-generating photosensitizer. Endosomal antig... More
