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Iron-induced transferrin receptor-1 mRNA destabilization: A response to "Neither miR-7-5p nor miR-141-3p is a major mediator of iron-responsive transferrin receptor-1 mRNA degradation".

RNA. 2019; 
Miyazawa M, Bogdan AR, Hashimoto K, Tsuji Y.
Products/Services Used Details Operation
Custom Vector Construction MATERIALS AND METHODS Luciferase assay The TfR1 reporters and mutations were synthesized at GenScript and cloned into the pMIRGLO luciferase vector (Promega), as previously described (Rupani and Connell 2016). Get A Quote

摘要

We read with great interest the Divergent Views article by Connell and colleagues disputing our recent publication describing a role for two microRNAs in the iron-mediated regulation of transferrin receptor 1 (TfR1) mRNA stability. Our publication sought to shed light on a long-standing question in the field of cellular iron metabolism, and we welcome commentary and critique. However, there are several critical issues contained in the article by Connell and colleagues that require further consideration. We appreciate the opportunity to reply here.,© 2019 Miyazawa et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

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