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Custom Vector Construction> | 4 SOURCE ATCC ATCC Genscript Clontech Addgene Addgene IDENTIFIER CCL-243 CRL-1855 NCBI ACCESSION# NM_015470.... Generation of a Rab11Fip5 stably expressing NK-92 cell line The human RAB11FIP5 gene was synthesized (Genscript), cloned into lentiviral vector pLVX-IRES-ZsGreen1 (Clontech,Catlog#632187), and co-transfected into 293FT cells with lentiviral packaging plasmids pMD2. | Get A Quote |
HIV-1 broadly neutralizing antibodies (bnAbs) are difficult to induce with vaccines but are generated in ∼50% of HIV-1-infected individuals. Understanding the molecular mechanisms of host control of bnAb induction is critical to vaccine design. Here, we performed a transcriptome analysis of blood mononuclear cells from 47 HIV-1-infected individuals who made bnAbs and 46 HIV-1-infected individuals who did not and identified in bnAb individuals upregulation of RAB11FIP5, encoding a Rab effector protein associated with recycling endosomes. Natural killer (NK) cells had the highest differential expression of RAB11FIP5, which was associated with greater dysregulation of NK cell subsets in bnAb subjects. NK cells f... More