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Identification and expression analyses of new genes associated with ciprofloxacin resistance in Vibrio parahaemolyticus.

Food Res Int. 2019;

Zhou H, Liang Y, Gao L, Ren J, Xue F, Guo D, Jiang Y, Yang Z, Lian L, Dai J.

Products/Services Used	Details	Operation
Gene Synthesis	5 16 83 83 123 83 83 83 83 Base change parC mutation Amino acid residue Position 85 AGT → ATT AGT → ATT ACC → ACA AGT → ATT AGT → ATT AGT → ATT AGT → ATT Base change TCT → TTT Mutation – – – – – – – – Ser → Phe – – – – – – – – – Mutation —c – – – – – – Ser → Ile Ser → Ile Thrd(—) – Ser → Ile Ser → Ile – – – – Ser → Ile Ser → Ile DNA bands of expected sizes were purified using a gel DNA recovery kit (AxyPrep, Axygen, USA) for sequencing (GenScript Biotech Co.	Get A Quote

摘要

Quinolone-resistant foodborne pathogens have become an important public health concern, however, little is known about the molecular mechanism of ciprofloxacin (CIP) resistance among Vibrio parahaemolyticus isolates. This study aimed to explore new genes implicated in resistance to CIP in genome-wide. CIP susceptibility of six V. parahaemolyticus isolates was analyzed by disk diffusion and micro-broth dilution methods. To establish a model for CIP-resistant V. parahaemolyticus, in vitro continuous subcultures in drug gradient medium were adopted, and minimum inhibitory concentrations (MICs) was eventually increased by 64-128 times. Quinolone resistance determining region (QRDR) genes were screened by polymerase... More