| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | … signalling domain The eGFP or EGFRt reporters were connected downstream of the construct using a T2A linker The whole construct was cloned into a pcDNA31(+) vector backbone (GenScript) Linearised templates were … | Get A Quote |
Chimeric antigen receptors (CARs) have found clinical success in B cell malignancies, but a dearth of potential targets limits their wider clinical application, especially in solid tumours. Here, we describe the development of an anti-annexin A2 CAR, CAR(2448), derived from an antibody found to have activity against epithelial ovarian cancer cell lines. The spacer length of CAR(2448) was optimised based on in vitro cytotoxic activity against ovarian cancer (OC) cell lines via a real-time cytotoxicity assay. The longer spacer CAR(2448)L T cells exhibit significant effector activity, inducing inflammatory cytokine release and cytotoxicity against OC cell lines. Furthermore, CAR(2448)L-BBz T cells induced enhanced... More
