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Critical role of ARID3B in the expression of pro-apoptotic p53-target genes and apoptosis.

Biochem Biophys Res Commun. 2015; 
Pratama E, Tian X, Lestari W, Iseki S, Ichwan SJ, Ikeda MA.
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Custom Vector Construction … To generate pEF-V5-ARID3B, ARID3B cDNA was synthesized (GenScript) and then cloned into the destination vector pEF-DEST51 (Invitrogen … The following primary antibodies were used in this study: mouse monoclonal anti-p53 (DO-1; Santa Cruz Biotechnology, Santa Cruz … Get A Quote

摘要

ARID3A and ARID3B are transcriptional targets of p53. Recently, it has been reported that ARID3A plays a critical role in the transcriptional activation of pro-arrest p21 in response to DNA damage. However, the role of ARID3B in the p53 regulatory pathway remains poorly understood. Here we show that ARID3A and ARID3B specifically bind to putative ARID3-binding sites in p53 target genes in vitro and in vivo. ARID3B and, to a lesser extent, ARID3A silencing blocked transcriptional activation of pro-apoptotic p53 target genes, such as PUMA, PIG3, and p53. Furthermore, ectopic ARID3B, to a lesser extent, ARID3A expression activated the pro-apoptotic gene expression, and only ARID3B induced apoptosis. Finally, ARI... More

关键词

ARID3A; ARID3B; Apoptosis; Gene expression; p53