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A pathway linking translation stress to checkpoint kinase 2 signaling in .

Proc. Natl. Acad. Sci. U.S.A.. 2019; 
DiernfellnerAxel C R,LauingerLinda,ShostakAnton,BrunnerMic
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Gene Synthesis The N-terminal multiple phosphorylation site mutants prd-4A and prd-4AQ were created by gene synthesis (Genscript; see SI Appendix for sequences). P Get A Quote

摘要

Checkpoint kinase 2 (CHK-2) is a key component of the DNA damage response (DDR). CHK-2 is activated by the PIP3-kinase-like kinases (PI3KKs) ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related protein (ATR), and in metazoan also by DNA-dependent protein kinase catalytic subunit (DNA-PKcs). These DNA damage-dependent activation pathways are conserved and additional activation pathways of CHK-2 are not known. Here we show that PERIOD-4 (PRD-4), the CHK-2 ortholog of , is part of a signaling pathway that is activated when protein translation is compromised. Translation stress induces phosphorylation of PRD-4 by a PI3KK distinct from ATM and ATR. Our data indicate that the activatin... More

关键词

Neurospora crassa,checkpoint kinase 2,circadian clock,mTOR,translation inhibi