| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | Synthetic DNA constructs were generated (GenScript) that spanned the step sequence between the restriction sites but replaced the sequence encoding the CC domain of Step (Fig 3B) with sequences encoding alternate peptides (maintaining the flanking proline residues shown for the Step CC in Fig 3B). | Get A Quote |
Tissue dynamics require regulated interactions between adherens junctions and cytoskeletal networks. For example, myosin-rich adherens junctions recruit the cytohesin Arf-GEF Steppke, which down-regulates junctional tension and facilitates tissue stretching. We dissected this recruitment mechanism with structure-function and other analyses of Steppke and Stepping stone, an implicated adaptor protein. During Drosophila dorsal closure, Steppke's coiled-coil domain was necessary and sufficient for junctional recruitment. Purified coiled-coil domains of Steppke and Stepping stone heterodimerized through a hydrophobic surface of the Steppke domain. This mapped surface was required for Steppke's junctional localizati... More
