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Agrobacterium-Mediated Transient Gene Expression in Developing Ricinus communis Seeds: A First Step in Making the Castor Oil Plant a Chemical Biofactory

Front Plant Sci.. 2019; 
Sánchez-Álvarez A1, Ruíz-López N2, Moreno-Pérez AJ1, Martínez-Force E1, Garcés R1, Salas JJ1.
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Gene Synthesis … NcoI fragment using specific primer pairs, into the GUS control vector with the same pair of<br> restriction enzymes (Supplementary Table 1). For the pCAMBIA 1305.1:pGlycinin::LfKCS3<br> construct the LfKCS3 open reading frame was re-synthesized (<b>GenScript</b> Corporation,) and … Get A Quote

摘要

The castor oil plant represents a promising platform to produce oils with industrial applications. However, its use in biotechnology is limited by the absence of a well-established procedure to transform it, and a poor understanding of gene regulation and promoter use in this species. As such, a method has been developed to express proteins or hairpin-RNA in this plant, a method based on the direct injection of Agrobacterium into the developing endosperm of castor oil fruit, enabling different constructs and promoters to be tested. This method produces a high rate of transformation and a good proportion of viable seeds that express reporter genes for up to 20 days after infiltration (DAI). Gene expression under... More

关键词

Lesquerella fendleri; Ricinus communis; acyl-CoA Pool; elongase; promoter; transient expression