| Products/Services Used | Details | Operation |
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| Gene Synthesis | The pGEM4Z-luc and pGEM4Z-EGFP plasmids used for the preparation of luciferase and EGFP RNA have been previously described.7 The self-replicative RNA plasmid encoding GFP, pT7-VEE-GFP was a gift from Steven Dowdy (Addgene plasmid #58977). This plasmid is derived from Venezuelan equine encephalitis virus.75HA from influenza was synthetized by Genscript (Piscataway, NJ, USA) and inserted into previously digested pT7-VEE-GFP to replace GFP by HA to obtain pT7-VEE-HA. | Get A Quote |
Nucleic acid vaccination relies on injecting DNA or RNA coding antigen(s) to induce a protective immune response. RNA vaccination is being increasingly used in preclinical and clinical studies. However, few delivery systems have been reported for in vivo delivery of RNA of different sizes. Using a tripartite formulation with RNA, cationic polymer, and anionic liposomes, we were able to encapsulate RNA into neutral lipopolyplexes (LPPs). LPPs were stable in vitro and successfully delivered conventional RNA and replicative RNA to dendritic cells in cellulo. Their injection led to reporter gene expression in mice. Finally, administration of LPP-Replicon RNA (RepRNA) led to an adaptive immune response against ... More
