| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | For intracellular detection of IFN-γ and TNF, CD8+ T cells were stimulated ex vivo with LCMV gp33-41 peptide (KAVYNFATM) (GenScript) in the presence of brefeldin A (Sigma) for 6 h at 37 °C; cells were stained with surface antibodies and then fixed in 4% paraformaldehyde (Electron Microscopy Services) and permeabi - lized before staining with intracellular antibodies. | Get A Quote |
During microbial infection， responding CD8 T lymphocytes differentiate into heterogeneous subsets that together provide immediate and durable protection. To elucidate the dynamic transcriptional changes that underlie this process， we applied a single-cell RNA-sequencing approach and analyzed individual CD8 T lymphocytes sequentially throughout the course of a viral infection in vivo. Our analyses revealed a striking transcriptional divergence among cells that had undergone their first division and identified previously unknown molecular determinants that controlled the fate specification of CD8 T lymphocytes. Our findings suggest a model for the differentiation of terminal effector cells initiated by an ear... More
