In organisms from insects to vertebrates， Toll-like receptors (TLRs) are primary pathogen detectors that activate downstream pathways， specifically those that direct expression of innate immune effector genes. TLRs also have roles in development in many species. The sea anemone is a useful cnidarian model to study the origins of TLR signaling because its genome encodes a single TLR and homologs of many downstream signaling components， including the NF-κB pathway. We have characterized the single TLR (Nv-TLR) and demonstrated that it can activate canonical NF-κB signaling in human cells. Furthermore， we show that the intracellular Toll/IL-1 receptor (TIR) domain of Nv-TLR can interact with the human ... More
In organisms from insects to vertebrates， Toll-like receptors (TLRs) are primary pathogen detectors that activate downstream pathways， specifically those that direct expression of innate immune effector genes. TLRs also have roles in development in many species. The sea anemone is a useful cnidarian model to study the origins of TLR signaling because its genome encodes a single TLR and homologs of many downstream signaling components， including the NF-κB pathway. We have characterized the single TLR (Nv-TLR) and demonstrated that it can activate canonical NF-κB signaling in human cells. Furthermore， we show that the intracellular Toll/IL-1 receptor (TIR) domain of Nv-TLR can interact with the human TLR adapter proteins MAL and MYD88. We demonstrate that the coral pathogen causes a rapidly lethal disease in and that heat-inactivated and bacterial flagellin can activate a reconstituted Nv-TLR-to-NF-κB pathway in human cells. By immunostaining of anemones， we show that Nv-TLR is expressed in a subset of cnidocytes and that many of these Nv-TLR-expressing cells also express Nv-NF-κB. Additionally， the nematosome， which is a -specific multicellular structure， expresses Nv-TLR and many innate immune pathway homologs and can engulf Morpholino knockdown indicates that Nv-TLR also has an essential role during early embryonic development. Our characterization of this primitive TLR and identification of a bacterial pathogen for reveal ancient TLR functions and provide a model for studying the molecular basis of cnidarian disease and immunity.
