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Synonymous codon usage affects the expression of wild type and F508del CFTR.

J. Mol. Biol.. 2015; 
Shah Kalpit,Cheng Yi,Hahn Brian,Bridges Robert,Bradbury Neil A,Mueller Dav
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Custom Vector Construction Synthetic CFTR constructs with High Codon Adaptive Index (hCAI), Low CAI (lCAI), Mosaic of High and Low CAI (mCAI) were commercially made (Genscript, Piscataway, 19 N. Get A Quote

摘要

The cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel composed of 1480 amino acids. The major mutation responsible for cystic fibrosis results in loss of amino acid residue, F508 (F508del). Loss of F508 in CFTR alters the folding pathway resulting in endoplasmic-reticulum-associated degradation. This study investigates the role of synonymous codon in the expression of CFTR and CFTR F508del in human HEK293 cells. DNA encoding the open reading frame (ORF) for CFTR containing synonymous codon replacements was expressed using a heterologous vector integrated into the genome. The results indicate that the codon usage greatly affects the expression of CFTR. While the promoter strength d... More

关键词

CFTR,CFTR F508del,nonsense mediated decay,protein folding,synonymous codon u