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Activation of Evi1 inhibits cell cycle progression and differentiation of hematopoietic progenitor cells.

Leukemia. 2013; 
Kustikova O S,Schwarzer A,Stahlhut M,Brugman M H,Neumann T,Yang M,Li Z,Schambach A,Heinz N,Gerdes S,Roeder I,Ha T C,Steinemann D,Schlegelberger B,Ba
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Custom Vector Construction 1),1 was equipped with a Kozak sequence, codon- optimized (Genscript Corporation, Piscatway, NJ, USA) removing cryptic splice and polyA sites to improve translation and mRNA half-life, and cloned via AgeI and SalI sites into a lentiviral self-inactivating (SIN) vector (pRRL. Get A Quote

摘要

The transcription factor Evi1 has an outstanding role in the formation and transformation of hematopoietic cells. Its activation by chromosomal rearrangement induces a myelodysplastic syndrome with progression to acute myeloid leukemia of poor prognosis. Similarly, retroviral insertion-mediated upregulation confers a competitive advantage to transplanted hematopoietic cells, triggering clonal dominance or even leukemia. To study the molecular and functional response of primary murine hematopoietic progenitor cells to the activation of Evi1, we established an inducible lentiviral expression system. EVI1 had a biphasic effect with initial growth inhibition and retarded myeloid differentiation linked to enha... More

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