| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The VH and VL of anti-CD3 (humanized UCHT1)20 and the camel anti-CEA VHH21 were directly synthesized (Genscript) and cloned into the pET21a or pET26b plasmid. | Get A Quote |
Bispecific antibodies that engage immune cells to kill cancer cells have been actively studied in cancer immunotherapy. In this study， we present a novel bispecific format， S-Fab， fabricated by linking a single-domain anti-carcinoembryonic antigen VHH to a conventional anti-CD3 Fab. In contrast to most bispecific antibodies， the S-Fab bispecific antibody can be efficiently expressed and purified from bacteria. The purified S-Fab is stable in serum and is able to recruit T cells to drive potent cancer cell killing. In xenograft models， the S-Fab antibody suppresses tumor growth in the presence of human immune cells. Our study suggested that the bispecific S-Fab format can be applied to a wide range of ... More
