Products/Services Used | Details | Operation |
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DNA Sequencing> | Correct orientation was verified by sequencing (GenScript), and the puri- fied plasmid was transfected into 293-6E cells grown in serum-free expression media (Life Technologies).... A prominent band of 40–44 kD was present, and detected as a single band in Western blot with antibody against human IgG (goat anti-human IgG–horseradish peroxidase [HRP]; GenScript). | Get A Quote |