| Products/Services Used | Details | Operation |
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| Peptide Synthesis | 05% w/v Irgacure 2959 photoinitiator (I2959; Ciba Specialty Chemicals), as well as a 2× molar excess of either head-to-tail cyclized Arg–Gly–Asp–[d-Phe]–Cys (cyclic RGD; Genscript) adhesion peptide, 2× molar excess of linear H–Cys–Arg– Gly–Asp–Ser–NH2 (linear RGD; Genscript), 2× molar excess of non-functional H–Cys–Arg–Asp–Gly–Ser–NH2 scrambled adhesion peptide (CRDGS; Genscript), 3× molar excess of H–Cys–Glu–[d-Phe]–[d-Ala]–[d-Tyr]–[d-Leu]–Iso–Asp– Phe–Asn–Trp–Glu–Tyr–Pro–Ala–Ser–Lys–NH2 (VBP) or 3× molar excess of the non-functional scrambled VBP peptide H–Cys–Asp–[d-Ala]–Pro–Tyr– Asn–[d-Phe]–Glu–Phe–Ala–Trp–Lys–[d-Tyr]–Iso–Ser–[d-Leu]–Glu–NH2.... The hydrogels used for assessing endothelial-cell-network formation consisted of PEGNB molecules, PEG molecules preconjugated to RGD adhesion peptides and VBP, MMP-degradable H–Lys–Cys–Gly–Gly–Pro–Gln–Gly–Ile–Trp–Gly–Gln–Gly–Cys–Lys–NH2 crosslinking peptide (Genscript) and 0. | Get A Quote |
The physiological relevance of Matrigel as a cell-culture substrate and in angiogenesis assays is often called into question. Here， we describe an array-based method for the identification of synthetic hydrogels that promote the formation of robust vascular networks for the detection of putative vascular disruptors， and that support human embryonic stem cell expansion and pluripotency. We identified hydrogel substrates that promoted endothelial-network formation by primary human umbilical vein endothelial cells and by endothelial cells derived from human induced pluripotent stem cells， and used the hydrogels with endothelial networks to identify angiogenesis inhibitors. The synthetic hydrogels show super... More
