| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Synchronized germinating embryos were selected and transferred to gel medium in glass test tubes containing scrambled pep- tide (sCLV3; 5 or 10 µM; Genscript) or ESR2c, ZmFCP1, AtCLV3, ZmCLE7, CLE20, CLE40, ZmCLE21, or ZmCLE23 peptide (peptide sequences are listed in Supplementary Table 3).... The precursors for artificial miRNAs against At3g25670 (Supplementary Table 2) were cloned into the miR-159a backbone follow- ing synthesis (Genscript) and were then mobilized into pDONR P4r-P3r using the Multisite Gateway System. | Get A Quote |
Shoot apical meristems are stem cell niches that balance proliferation with the incorporation of daughter cells into organ primordia. This balance is maintained by CLAVATA-WUSCHEL feedback signaling between the stem cells at the tip of the meristem and the underlying organizing center. Signals that provide feedback from organ primordia to control the stem cell niche in plants have also been hypothesized， but their identities are unknown. Here we report FASCIATED EAR3 (FEA3)， a leucine-rich-repeat receptor that functions in stem cell control and responds to a CLAVATA3/ESR-related (CLE) peptide expressed in organ primordia. We modeled our results to propose a regulatory system that transmits signals from diff... More
