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Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond.

Science. 2017; 
MitchellLeslie A,WangAnn,StracquadanioGiovanni,KuangZheng,WangXuya,YangKun,RichardsonSarah,MartinJ Andrew,ZhaoYu,WalkerRoy,LuoYisha,DaiHongjiu,DongKang,TangZuojian,YangYanling,CaiYizhi,HeguyAdriana,UeberheideBeatrix,FenyöDavid,DaiJunbiao,BaderJoel S,BoekeJ
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Custom Vector Construction 8GenScript, Piscataway, NJ 08854, USA.... are employees of GenScript, who constructed most of the synVI synthetic DNA chunks. Get A Quote

摘要

We describe design, rapid assembly, and characterization of synthetic yeast Sc2.0 chromosome VI (synVI). A mitochondrial defect in the synVI strain mapped to synonymous coding changes within (), encoding an essential proteasome subunit; Sc2.0 coding changes reduced Pre4 protein accumulation by half. Completing Sc2.0 specifies consolidation of 16 synthetic chromosomes into a single strain. We investigated phenotypic, transcriptional, and proteomewide consequences of Sc2.0 chromosome consolidation in poly-synthetic strains. Another "bug" was discovered through proteomic analysis, associated with alteration of the transcription start due to transfer RNA deletion and loxPsym site insertion. Despite ex... More

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