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Crystal structure of E. coli PRPP synthetase.

BMC Struct. Biol.. 2019;

ZhouWeijie，TsaiAndrew，DattmoreDevon A，StivesDevin P，ChitrakarIva，D'alessandroAlexis M，PatilShiv，HicksKatherine A，FrenchJarr

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Gene Synthesis	1) was synthesized (GenScript) and cloned as an N-terminally His-tagged protein into the pTHT vector (a modified form of pET-28 with a Tobacco Etch Virus protease site in place of the thrombin site [24]) and expressed in E coli BL21(DE3) strain.	Get A Quote

摘要

Ribose-phosphate pyrophosphokinase (EC 2.7.6.1) is an enzyme that catalyzes the ATP-dependent conversion of ribose-5-phosphate to phosphoribosyl pyrophosphate. The reaction product is a key precursor for the biosynthesis of purine and pyrimidine nucleotides.