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Prediction and characterization of promoters and ribosomal binding sites of in system biology era.

Biotechnol Biofuels. 2019; 
YangYongfu,ShenWei,HuangJu,LiRunxia,XiaoYubei,WeiHui,ChouYat-Chen,ZhangMin,HimmelMichael E,ChenShouwen,YiLi,MaLixin,YangSh
Products/Services Used Details Operation
Gene Synthesis Briefly, highly purified salt-free primer pairs for EGFP and opmCherry were synthesized (Genscript, China) with an similar annealing temperature of 60 °C (Additional file 1: Table S1). Get A Quote

摘要

is a model bacterial ethanologen with many systems biology studies reported. Besides lignocellulosic ethanol production, has been developed as a platform for biochemical production through metabolic engineering. However, identification and rigorous understanding of the genetic origins of cellular function, especially those based in non-coding region of DNA, such as promoters and ribosomal binding sites (RBSs), are still in its infancy. This knowledge is crucial for the effective application of to new industrial applications of biotechnology for fuels and chemicals production.

关键词

Dual reporter-gene system,Promoter,Reporter genes,Ribosomal binding site (RBS),Systems biology,Zymomonas mob