Products/Services Used | Details | Operation |
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Custom Vector Construction> | pseudomallei LptB, optimized for expression in Escherichia coli (GenScript), was subcloned into the expression vector pET-15b-TEV (modified from pET-15b; Novagen). | Get A Quote |
The thick outer membrane (OM) of Gram-negative bacteria performs an important protective role against hostile environments, supports cell integrity, and contributes to surface adhesion and in some cases also to virulence. A major component of the OM is lipopolysaccharide (LPS), a complex glycolipid attached to a core containing fatty-acyl chains. The assembly and transport of lipid A, the membrane anchor for LPS, to the OM begins when a heteromeric LptBFG protein complex extracts lipid A from the outer leaflet of the inner membrane. This process requires energy, and upon hydrolysis of ATP one component of the heteromeric assembly, LptB, triggers a conformational change in LptFG in support of lip... More