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Selective sensitization of human neutrophils to LukGH mediated cytotoxicity by Staphylococcus aureus and IL-8.

J. Infect.. 2017;

Janesch Philipp，Rouha Harald，Weber Susanne，Malafa Stefan，Gross Karin，Maierhofer Barbara，Badarau Adriana，Visram Zehra C，Stulik Lukas，Nagy Es

Products/Services Used	Details	Operation
Molecular Biology Reagents	Endotoxin content was measured for several toxin batches using the chromogenic Limulus Amebocyte Lysate (LAL Chromogenic Endotoxin Quantification kit, Pierce or ToxinSensorTM Chromogenic LAL Endotoxin Assay Kit,Genscript).	Get A Quote

摘要

Staphylococcus aureus produces up to five bi-component leukocidins - LukSF-PV， gamma-hemolysins AB and CB， LukGH (LukAB) and LukED - to evade innate immunity by lysing phagocytic cells. Species specificity of these leukocidins limits the relevance of animal models， therefore we assessed their individual contribution using human neutrophils.