is a facultative anaerobe and is one of the most widely studied bacterial strains because of its ability to use a variety of substrates， to produce hydrogen at a high rate， and its high growth rate during dark fermentation. However， the rate of hydrogen production has not been optimized. In this present study， three strategies to improve hydrogen production in ， namely the disruption of ， overexpression of the small RNA RyhB and of NadE to regulate global anaerobic metabolism， and the redistribution of metabolic flux. The goal of this study was to clarify the effect of ， RyhB， and NadE on hydrogen production and how the perturbation of NADH influences the yield of hydrogen gas from .
is a facultative anaerobe and is one of the most widely studied bacterial strains because of its ability to use a variety of substrates， to produce hydrogen at a high rate， and its high growth rate during dark fermentation. However， the rate of hydrogen production has not been optimized. In this present study， three strategies to improve hydrogen production in ， namely the disruption of ， overexpression of the small RNA RyhB and of NadE to regulate global anaerobic metabolism， and the redistribution of metabolic flux. The goal of this study was to clarify the effect of ， RyhB， and NadE on hydrogen production and how the perturbation of NADH influences the yield of hydrogen gas from .
