| Products/Services Used | Details | Operation |
|---|---|---|
| DNA Sequencing | A total number of 160 positive clones (40 clones for each type of fish) were tested by PCR and then sequenced by Nanjing Genscript. | Get A Quote |
The allotetraploid hybrid fish (4nAT) that was created in a previous study through an intergeneric cross between red crucian carp (Carassius auratus red var.， ♀) and common carp (Cyprinus carpio L.， ♂) provided an excellent platform to investigate the effect of hybridization and polyploidization on the evolution of 5S rDNA. The 5S rDNAs of paternal common carp were made up of a coding sequence (CDS) and a non-transcribed spacer (NTS) unit， and while the 5S rDNAs of maternal red crucian carp contained a CDS and a NTS unit， they also contained a variable number of interposed regions (IPRs). The CDSs of the 5S rDNAs in both parental fishes were conserved， while their NTS units seemed to have been sub... More
