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A high-throughput expression screening platform to optimize the production of antimicrobial peptides.

Microb. Cell Fact.. 2017; 
Schreiber Christine,Müller Hagen,Birrenbach Oliver,Klein Moritz,Heerd Doreen,Weidner Tobias,Salzig Denise,Czermak P
Products/Services Used Details Operation
Molecular Biology Reagents The plasmid features were synthesized by GenScript. Get A Quote

摘要

Antimicrobial peptides (AMPs) are promising candidates for the development of novel antibiotics, but it is difficult to produce sufficient quantities for preclinical and clinical studies due to their toxicity towards microbial expression hosts. To avoid laborious trial-and-error testing for the identification of suitable expression constructs, we have developed a small-scale expression screening platform based on a combinatorial plasmid library.

关键词

Antimicrobial peptides,Combinatorial library,E. coli,Expression screening,Expression strain,Fusion protein,Golden Gate cloning,P. pastoris,Promoter,Recombinant protein produc