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Development of a high-throughput opsonophagocytic assay for the determination of functional antibody activity against Streptococcus pyogenes using bioluminescence.

J. Microbiol. Methods. 2017;

Lorenz Natalie，Loh Jacelyn M S，Moreland Nicole J，Proft Th

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Catalog Peptides	ELISA analysis of IVIG revealed reactivity against strain-specific hypervariable region peptides of the M proteins of GAS serotypes M1, M6 and M89 (aa1-50 of the corresponding mature M protein [M1 SF370, M6 MGAS10394, M89 GAS131361], chemically synthesised by GenScript USA Inc) (Figure 2A).	Get A Quote

摘要

The lack of standardised protocols for the assessment of functional antibodies has hindered Streptococcus pyogenes research and the development of vaccines. A robust， high throughput opsonophagocytic bactericidal assay to determine protective antibodies in human and rabbit serum has been developed that utilises bioluminescence as a rapid read out.