至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Development of a colourimetric assay for glycosynthases.

J. Biotechnol.. 2017; 
Hayes Marc R,Bochinsky Kevin A,Seibt Lisa S,Elling Lothar,Pietruszka J
Products/Services Used Details Operation
Custom Vector Construction ) was synthesised chemically (GenScript) in a pET21a(+) expression vector also between the NdeI Get A Quote

摘要

The synthesis of glycosidic structures by catalysis via glycosynthases has gained much interest due to the potential high product yields and specificity of the enzymes. Nevertheless, the characterisation and implementation of new glycosynthases is greatly hampered by the lack of high-throughput methods for reaction analysis and screening of potential glycosynthase variants. Fluoride detection, via silyl ether chemosensors, has recently shown high potential for the identification of glycosynthase mutants in a high-throughput manner, though limited by the low maximal detection concentration. In the present paper, we describe a new version of a glycosynthase activity assay using a silyl ether of p-nitrop... More

关键词

Chemosensor,Enzyme characterisation,Fluoride detection,Glycosynthase,Scree