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AMD1 mRNA employs ribosome stalling as a mechanism for molecular memory formation.

Nature. 2018; 
Yordanova Martina M,Loughran Gary,Zhdanov Alexander V,Mariotti Marco,Kiniry Stephen J,O'Connor Patrick B F,Andreev Dmitry E,Tzani Ioanna,Saffert Paul,Michel Audrey M,Gladyshev Vadim N,Papkovsky Dmitry B,Atkins John F,Baranov Pav
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摘要

In addition to acting as template for protein synthesis, messenger RNA (mRNA) often contains sensory sequence elements that regulate this process. Here we report a new mechanism that limits the number of complete protein molecules that can be synthesized from a single mRNA molecule of the human AMD1 gene encoding adenosylmethionine decarboxylase 1 (AdoMetDC). A small proportion of ribosomes translating AMD1 mRNA stochastically read through the stop codon of the main coding region. These readthrough ribosomes then stall close to the next in-frame stop codon, eventually forming a ribosome queue, the length of which is proportional to the number of AdoMetDC molecules that were synthesized from the same AMD1 ... More

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