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Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28.

Mol. Cell. 2017; 
Smargon Aaron A,Cox David B T,Pyzocha Neena K,Zheng Kaijie,Slaymaker Ian M,Gootenberg Jonathan S,Abudayyeh Omar A,Essletzbichler Patrick,Shmakov Sergey,Makarova Kira S,Koonin Eugene V,Zhang
Products/Services Used Details Operation
Custom Vector Construction (GenScript) and inserted into a bacterial expres- sion vector (6x His/Twin Strep SUMO, a pET based vector Get A Quote

摘要

CRISPR-Cas adaptive immune systems defend microbes against foreign nucleic acids via RNA-guided endonucleases. Using a computational sequence database mining approach, we identify two class 2 CRISPR-Cas systems (subtype VI-B) that lack Cas1 and Cas2 and encompass a single large effector protein, Cas13b, along with one of two previously uncharacterized associated proteins, Csx27 and Csx28. We establish that these CRISPR-Cas systems can achieve RNA interference when heterologously expressed. Through a combination of biochemical and genetic experiments, we show that Cas13b processes its own CRISPR array with short and long direct repeats, cleaves target RNA, and exhibits collateral RNase activity. Us... More

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