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Activator Protein-1: redox switch controlling structure and DNA-binding.

Nucleic Acids Res.. 2017; 
Yin Zhou,Machius Mischa,Nestler Eric J,Rudenko G
Products/Services Used Details Operation
Custom Vector Construction JunD bZIP domain (residues Q266 -V332 ) was cloned into a pET21a ex- pression vector (Genscript Get A Quote

摘要

The transcription factor, activator protein-1 (AP-1), binds to cognate DNA under redox control; yet, the underlying mechanism has remained enigmatic. A series of crystal structures of the AP-1 FosB/JunD bZIP domains reveal ordered DNA-binding regions in both FosB and JunD even in absence DNA. However, while JunD is competent to bind DNA, the FosB bZIP domain must undergo a large conformational rearrangement that is controlled by a 'redox switch' centered on an inter-molecular disulfide bond. Solution studies confirm that FosB/JunD cannot undergo structural transition and bind DNA when the redox-switch is in the 'OFF' state, and show that the mid-point redox potential of the redox switch affords it s... More

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