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A protocol combining multiphoton microscopy and propidium iodide for deep 3D root meristem imaging in rice: application for the screening and identification of tissue-specific enhancer trap lines.

Plant Methods. 2018; 
Bureau Charlotte,Lanau Nadège,Ingouff Mathieu,Hassan Boukhaddaoui,Meunier Anne-Cécile,Divol Fanchon,Sevilla Rosie,Mieulet Delphine,Dievart Anne,Périn Christ
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Molecular Biology Reagents via synthesis and cloned into the plasmid pUC57 (Genscript). Native recognition sites for the BamHI Get A Quote

摘要

The clear visualization of 3D organization at the cellular level in plant tissues is needed to fully understand plant development processes. Imaging tools allow the visualization of the main fluorophores and in vivo growth monitoring. Confocal microscopy coupled with the use of propidium iodide (PI) counter-staining is one of the most popular tools used to characterize the structure of root meristems in . However, such an approach is relatively ineffective in species with more complex and thicker root systems.

关键词

CFP,GFP,Multiphoton microscope,Propidium iodide,Rice,Root meristem