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High specificity and sensitivity of Zika EDIII-based ELISA diagnosis highlighted by a large human reference panel.

PLoS Negl Trop Dis. 2019; 
Denis Jessica,Attoumani Sarah,Gravier Patrick,Tenebray Bernard,Garnier Annabelle,Briolant Sébastien,de Laval Franck,Chastres Véronique,Grard Gilda,Leparc-Goffart Isabelle,Coutard Bruno,Badaut C
Products/Services Used Details Operation
Gene Synthesis by GenScript. The sequences were then cloned into pET-24a or pET-19b plasmids (Novagen Get A Quote

摘要

Zika virus (ZIKV) and Dengue virus (DENV) are often co-endemic. The high protein-sequence homology of flaviviruses renders IgG induced by and directed against them highly cross-reactive against their antigen(s), as observed on a large set of sera, leading to poorly reliable sero-diagnosis.

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