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Ribosome-dependent Vibrio cholerae mRNAse HigB2 is regulated by a β-strand sliding mechanism.

Nucleic Acids Res.. 2017; 
Had?i San,Garcia-Pino Abel,Haesaerts Sarah,Jurenas Dukas,Gerdes Kenn,Lah Jurij,Loris
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Custom Vector Construction –104) was cloned by a commercial sup- plier (GenScript) into the pET21b+ vector (Novagen) us- ing Get A Quote

摘要

Toxin-antitoxin (TA) modules are small operons involved in bacterial stress response and persistence. higBA operons form a family of TA modules with an inverted gene organization and a toxin belonging to the RelE/ParE superfamily. Here, we present the crystal structures of chromosomally encoded Vibrio cholerae antitoxin (VcHigA2), toxin (VcHigB2) and their complex, which show significant differences in structure and mechanisms of function compared to the higBA module from plasmid Rts1, the defining member of the family. The VcHigB2 is more closely related to Escherichia coli RelE both in terms of overall structure and the organization of its active site. VcHigB2 is neutralized by VcHigA2, a modular pr... More

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