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Gene Synthesis> | PhADT3 was immunoprecipitated from 0.5 g of petals of 2-day-old wild-type flowers harvested at 10:00 h using rabbit anti-PhADT3 antibodies and Pierce™ Classic IP Kit (Thermo Scientific) according to the manufacturer’s protocol. Rabbit anti-PhADT3 polyclonal antibodies were generated against a synthetic PhADT3 peptide CFKTSIVFAHDKGTS and purified by cross-absorption with PhADT1-specific peptide CFKTSIVFAHEGTGV to increase antibodies specificity and prevent cross-reactivity with PhADT1 (GenScript, Piscataway, NJ). | Get A Quote |
In addition to being a vital component of proteins, phenylalanine is also a precursor of numerous aromatic primary and secondary metabolites with broad physiological functions. In plants phenylalanine is synthesized predominantly via the arogenate pathway in plastids. Here, we describe the structure, molecular players and subcellular localization of a microbial-like phenylpyruvate pathway for phenylalanine biosynthesis in plants. Using a reverse genetic approach and metabolic flux analysis, we provide evidence that the cytosolic chorismate mutase is responsible for directing carbon flux towards cytosolic phenylalanine production via the phenylpyruvate pathway. We also show that an alternative transcript... More