| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | Individual modules of the system in the constructs presented in this work were either PCR amplified from Addgene plasmid numbers 60903 and 60904 (gifts from Dr. Ron Vale, University of California, San Francisco) or were synthesized by GenScript. | Get A Quote |
Understanding genomic functions requires site-specific manipulation of loci via efficient protein effector targeting systems. However， few approaches for targeted manipulation of the epigenome are available in plants. Here， we adapt the dCas9-SunTag system to engineer targeted gene activation and DNA methylation in Arabidopsis. We demonstrate that a dCas9-SunTag system utilizing the transcriptional activator VP64 drives robust and specific activation of several loci， including protein coding genes and transposable elements， in diverse chromatin contexts. In addition， we present a CRISPR-based methylation targeting system for plants， utilizing a SunTag system with the catalytic domain of the Nicotian... More
